Specifications

Data Management

• Dedicated workstation
• 0–262,143 channels
• 18-bit resolution with IEEE 32-bit floating-point standard

Fluorescence Compensation

• Unlimited inter-beam and intra-beam compensation

Pulse Processing

Measurements include:

• Height (H)
• Area (A)
• Width (W)

for every parameter.

Time can be correlated with any parameter for kinetic experiments and other applications.

Threshold

Thresholds can be defined for any parameter from any laser source. Multiple thresholds can be simultaneously configured across one or multiple lasers.

Sample Flow Rate

The digital acquisition system processes more than 10,000 events/second.

Sampling System

• 40-position tube loader
• Throughput: 56 minutes per carousel using BD Multi-Check control cells

Optical Excitation

The instrument incorporates a gel-coupled quartz flow cell aligned with the laser optics to maximize excitation efficiency and fluorescence collection. Emitted fluorescence is collected through fiber optics and directed to octagonal and trigon detection assemblies, allowing simultaneous detection of fluorochromes with identical emission wavelengths. Reflective optical filters are employed instead of conventional transmission filters.

Light Sources / Lasers

• Blue: 488 nm
• Red: 633 nm
• Violet: 405 nm

Supports detection of:

• 8 fluorochromes
• FSC
• SSC

Specifically:

• FSC is detected with a photodiode.
• SSC is detected with a photomultiplier tube using the 488 nm laser.